YED

Yeast Extract Dextrose

Remember to autoclave an appropriate sized graduated cylinder to measure the glucose solution in the sterile hood.

To make	YED agar plates
at	mm plate diameter
pour	mL per plate
start with:	mL water
add:	g yeast extract, stir till dissolved
add:	g agar, leave stir bar in
autoclave for:	minutes
add asceptically	mL filter sterilized 20% glucose
for a final volume of	mL

Mark with a single red line.