YED

Yeast Extract Dextrose

For Bio 284

Remember to autoclave an appropriate sized graduated cylinder to measure the glucose solution in the sterile hood.

NOTE: 900 mL may boil over in a 1 L bottle in the autoclave. Try:

• Autoclave:
  • YE + agar in 800 mL
  • 100 mL H₂O
  • mix in BSC
• Add 100 mL sterile 20% glucose
• mix and pour

To make	YED agar plates
at	mm plate diameter
pour	mL per plate
start with:	mL water
add:	g yeast extract, stir till dissolved
add:	g agar, leave stir bar in
autoclave for:	minutes
add asceptically	mL filter sterilized 20% glucose
for a final volume of	mL

Mark with a single red line.