| 1&2 |
1/23 |
10 |
LL parental |
fixed in paraglut |
phalloidin |
learn about scope |
| 3&4 |
1/30 |
10 |
LL parental |
fixed in paraglut |
tubulin, phalloidin, DAPI |
NA |
| 5 |
2/6 |
10 |
LL parental (dense) |
fixed in paraglut |
tubulin, phalloidin, DAPI |
photobleaching |
| 6 |
2/8 |
none |
|
|
|
Image J, photoshop |
| 7 |
2/13 |
30 |
LL parental |
fixed in paraglut |
unstained |
immunofluorescence |
|
|
10 |
3t3 |
fixed in paraglut |
unstained |
|
|
|
10 |
LL parental |
live on coverslip |
|
to fix and stain |
| 8 |
2/15 |
|
|
|
|
Lab practical |
| 9 |
2/20 |
3 |
LL parental |
fixed in MeOH |
gamma tubulin 1:100 (mouse) (C) |
organelle ID lab |
|
|
3 |
LL parental |
fixed in MeOH |
Hec1 1:200 (mouse) (didn't do) |
|
|
|
3 |
LL parental |
fixed in MeOH |
LAP2 1:200 (mouse) (B) |
|
|
|
3 |
LL parental |
formaldehyde only |
Alpha actinin (mouse IgM) 1:200 (D) |
|
|
|
3 |
LL parental |
formaldehyde only |
Golgi 58K 1:100 (mouse) (A) |
|
|
|
3 |
LL parental |
doesn't matter |
secondary Ab only |
|
| 9 |
2/22 |
10 |
LL a-tub GFP/RFP myosin |
live in dish |
|
imaging live cells |
|
|
18 |
LL |
live in dish |
|
for mitotracker or ceramide |
|
|
10 |
LL H2B m cherry |
live in dish |
|
transfected unknowns |
| 10 |
2/27 |
|
|
|
|
GFP E. coli, motile E. coli, Volvox Carolina 152655, Tetrahymena Carolina 131620, Dictyostelium Carolina 55996, Paramecium caudatum Carolina 131554, Chaos (Pelomyxa) carolinensis Carolina 131324 |
| 11 |
3/1 |
10 |
3t3 |
live in dish |
|
motility |
|
|
10 |
LL parental |
live in dish |
|
|
|
|
10 |
melanoma |
live in dish |
|
|
|
|
10 |
LL GFP-actin |
live in dish |
|
|
| 12 |
3/6 |
9 |
3t3 |
live in dish |
|
Motility II |
|
|
12 |
LL GFP-tub |
|
|
|
|
|
3 |
LL GFP actin |
|
|
|
|
|
5 |
B16 |
|
|
|
| 13 |
3/8 |
|
|
|
|
Posters |
| 14 |
3/20 |
26 |
3t3 |
live on coverslip |
|
Endocytosis I |
|
3/22 |
18 |
3T3 |
live on coverslip |
|
Endocytosis II |
|
|
10 |
LL parental |
live on coverslip |
|
|
|
|
18 |
LL a-tub-GFP |
live on coverslip |
|
|
| 13 |
3/27 |
28 |
3t3 |
live in dish (dense) |
|
Signalling 1 |
|
3/29 |
28 |
3t3 |
live in dish (dense) |
|
Signalling 2 |
| 14 |
4/3 |
10 |
LL-a-tub-GFP |
live in dish |
|
mitosis, cytokinesis |
|
|
18 |
LL myo tub |
live in dish |
|
|
| 15 |
4/5 |
10 |
LL- parental |
live on coverslip |
|
for fixation |
|
|
10 |
LL GFP-tub (or tub & myo) |
live in dish |
|
for treatment, observation |