Mitotracker
Mitotracker kdorfman Wed, 02/21/2018 - 18:59Mitotracker GREEN
Mitotracker GREEN kdorfman Thu, 10/24/2019 - 20:34Excitation 490 nm : Emission 516 nm
MW: 674
50 ug in vial.
Add 74 uL DMSO to make 1 mM stock.
working concentration =~ 25 - 200 nM
1 uL 1mM stock into 10 mL medium makes 100 nM treatment solution
Mitotracker RED
Mitotracker RED kdorfman Thu, 10/24/2019 - 20:34Invitrogen M7512
https://www.thermofisher.com/order/catalog/product/M7513?SID=srch-srp-M…
Ex = 579 nm; Em = 599
50 ug per tube.
MW = 531
Add 100 uL DMSO to 50 ug in tube. Makes ~ 1 mM stock solution.
Use at 25 - 100 nM. (1 uL per 10 mL for 100 nM)
Dilute in medium. Treat with pre-warmed stain solution for 15 - 45 minutes. Replace staining solution with fresh (warm) medium.
To fix: use 3.7% formaldehyde (in medium); 37C for 15 minutes (??)