Nocodazole

Prevents polymerization of microtubules. Prevents cells from entering metaphase.

• Acros 358240100, 10 mg
  

• Soluble in DMSO

• Dilute stock in medium to treat cells.

• Make a 10 mg/mL (=33mM) stock: Add 1 mL DMSO to 10 mg in bottle.

• Aliquot 3 µL 33 mM per tube.

• Working concentration range is usually 100nM - 30 µM.

• add 97 µL DMSO to tube to make 100 µL of 1 mM

• 33 µL 1mM + 10 mL medium = 3.3 µM