Fluo-4 AM
Fluo-4 AM kdorfman Thu, 04/26/2018 - 18:04Fluo-4 stock
Invitrogen F14217 500 µL
Calcium indicator (fluoresces when bound to Calcium ions)
Ex 494 nm; Em 516 nm
1mM in DMSO
Protect from light
Store in dissicator.
20 µL aliquots. Each makes 10 mL of 2 µM solution
Fluo-4 staining solution
2 µM Fluo-4 + 0.02% pluronic in HBS
Incubate 15 - 60 min at 20 - 37C. Wash before viewing.
plus HBS to final volume