Alexa Fluor 568 Phalloidin

Fisher or Invitrogen A 12380

Binds to F-Actin

Ex/Em 578/600

Stock Solution: Add 1.5 mL MeOH to vial (~6.6µM)

Aliquots

• 8 µL stock per 0.5 mL tube
• Label: Add 400 µL PBS (or PBS 1% BSA), use 50µL per coverslip

Fix cells with formaldehyde

Procedure:

• 50 uL phalloidin on parafilm in humid chamber
• coverslip cell side down onto drop
• 15 - 20 minutes at room temp
• rinse repeatedly in PBS-Tween-azide
• blot the corner on a kimwipe
• Mount on a drop of mounting medium on a slide

In histology and bioimaging freezers

Thermo Fisher R415

From the manufacturer:

Rhodamine phalloidin is a high-affinity F-actin probe conjugated to the red-orange fluorescent dye, tetramethylrhodamine (TRITC).

• Selectively stains F-actin
• Excitiation/Emission: 540/565 nm
• Superior to antibody staining
• Optimal for fixed and permeabilized samples
• Very widely cited fluorescent phalloidin conjugate

In 262A freezer

Thermo Fisher T7471

From the manufacturer:

Texas Red®-X phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, red fluorescent Texas Red®-X dye.

• Selectively stains F-actin
• Outstanding fluorescence performance
• Excitation/Emission: 591/608 nm
• Superior to antibody staining
• Optimal for fixed and permeabilized samples

In histology freezer