Hormones & Inhibitors, etc.Hormones & Inhibitors, etc. kdorfman Wed, 06/20/2018 - 21:25
By compoundBy compound kdorfman Mon, 04/23/2018 - 17:49
ATPATP kdorfman Mon, 04/23/2018 - 17:50
10 mM ATP Stock
- 60 mg ATP in 8 mL H2O
- pH to 7.0 with 0.1 M NaOH
- Bring volume to 10 mL with H2O
- Aliquot and freeze.
AngiotensinAngiotensin kdorfman Thu, 04/26/2018 - 17:10
Sigma A9525 1mg/mL in water
Soluble in water 25 mg/mL (only use sterile water!)
MW: 1046.18 Asp-Arg-Val-Tyr-Ile-His-Pro-Phe
Subject to degradation in freezer storage - wear gloves!
Make 1 mg/mL stock (= 1.0462 mM)
Working concentration 1 µM
Dissolve 1:1000 in HBS
BP-ABP-A kdorfman Mon, 03/22/2021 - 18:11
100 mM stock in DMSO = 0.023g/mL
BP-SBP-S kdorfman Mon, 03/22/2021 - 18:12
MW = 250.27
100 mM stock in DMSO = 0.025g/mL
BlebbistatinBlebbistatin kdorfman Mon, 04/23/2018 - 17:49
(-)-Blebbistatin Sigma B05650-1mg
Info from Cayman:
A stock solution may be made by dissolving the (±)-blebbistatin in an organic solvent purged with an inert gas. (±)-Blebbistatin is soluble in organic solvents such as DMSO and dimethyl formamide (DMF). The solubility of (±)-blebbistatin in these solvents is approximately 10 mg/mL.
(±)-Blebbistatin is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, (±)-blebbistatin should first be dissolved in DMF and then diluted with the aqueous buffer of choice. (±)-Blebbistatin has a solubility of approximately 0.5 mg/ml in a 1:1 solution of DMF:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.
(±)-Blebbistatin is a selective cell-permeable inhibitor of non-muscle myosin II ATPases.1,2 It rapidly and reversibly inhibits Mg-ATPase activity and in vitro motility of non-muscle myosin IIA and IIB for several species (IC50 = 0.5-5.0 μM), while poorly inhibiting smooth muscle myosin (IC50 = 80 μM).3 Through these effects, blebbistatin blocks apoptosis-related bleb formation, directed cell migration and cytokinesis in vertebrate cells. Blebbistatin is inactivated by UV light,4 which may be particularly important in fluorescent cell imaging applications.
Note green fluorescent crystals seen in a 75µM solution.
Stock was 1 mg/mL in DMSO
44 µL 1mg/mL blebbistatin in DMSO in 1 mL non-CO2 medium
1 mg in 34 µL DMSO = ~100mM
MW = 292
Use at ~150 µM
BradykininBradykinin kdorfman Mon, 04/23/2018 - 18:56
- 9-amino acid peptide chain: Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
Bradykinin raises internal calcium levels
*Sigma B3259 - 1 mg septum bottle
MW = 1060.2
1 mg/mL stock
(= 1.0602 mM)
Add 1 mL H2O to the 1 mg in the bottle (NO CALCIUM! - some will be used for the calcium experiments in Lab 7.2)
Aliquots are 21.2 µL.
2 µM solution for use
Brefeldin ABrefeldin A kdorfman Tue, 11/12/2019 - 20:48
Fisher 00-4506 1000x solution
Keep in refrigerator
Brefeldin A is an inhibitor of intracellular protein transport. Incubation of cells in culture with Brefeldin A leads to blockade of protein transport to the Golgi complex (GC) and accumulation of proteins in the endoplasmic reticulum (ER). Addition of Brefeldin A during the last hours of in vitro activation of cells results in enhanced detection of intracellular cytokines. Brefeldin A is effective for enhanced detection of a majority of mouse and human intracellular cytokines; however, it is advised that the investigators evaluate the use and efficacy of this reagent as well as other protein transport inhibitors such as Monensin in their specific assay system.
The Brefeldin A Solution is supplied at 1000X working concentration in methanol. Prior to addition to the cell cultures, a 1:1000 dilution in the culture media should be made. The final working concentration of the Brefeldin A (at 1x) is 3.0 ug/ml.
CaffeineCaffeine kdorfman Tue, 04/13/2021 - 15:25
Soluble 10 mg/mL in water (with heat and stirring)
Make a 50mM (=0.05M) solution: 9.71mg/mL
CannabinolCannabinol kdorfman Fri, 05/11/2018 - 16:11
Tetrahydrocannabinol solution 1 mg/mL in methanol
store at 4C
Concanavalin AConcanavalin A kdorfman Mon, 04/23/2018 - 18:21
- Sigma C5275 5 mg
- Plant mitogen
- Lectin (carbohydrate-binding protein) extracted from the jack-bean, Canavalia ensiformis
Con A binds specifically to α-Mannose, α-Galactose structures found in sugars, glycoproteins and glycolipids
Make stock 5 mg/mL solution (add 1 mL sterile PBS to bottle – gently rotate to mix)
Keep sterile - work in sterile hood
aliquot 100 µL into microfuge tubes (cannot take repeated freeze-thaw cycles)
Con A coverslipsCon A coverslips kdorfman Sun, 05/24/2020 - 15:38
*Requirements:** * Acid washed coverslips stored in 100% ethanol.
ConA 0.5 mg/mL.
Large petridishes to store the coverslips.
Flame the acid-washed coverslips and cool for 10 sec. (Make 9-‐14 coverslips at a time to save time.)
Place the coverslips on top of the large petri dish.
- Thaw the conA from the freezer and dilute to 0.5 mg/mL
apply around 400uL the first two coverslips
Wait for 10 sec.
Tilt the coverslips and suck out the excess ConA
Repeat the same for all the coverslips.
Cover the petri dish with another lid of petri dish and allow a small opening to dry.
If possible,use a vacuum pump to dry it faster.
The process usually takes 15 minutes and drying takes half an hour.
* In a petri dish, place the coverslips on top of a clean kimwipe with the conA surface facing up.
Cover with the lid. 10 min before needed, UV sterilize them.
Alternatively, UV sterilize all of them after drying and open the dishes only in aseptic conditions or inside hood.
Con A dishesCon A dishes kdorfman Sun, 05/24/2020 - 15:37
Concanavalin-coated coverslip dishes
Start with 5 mg/mL ConA stock solution
Keep sterile - work in sterile hood
aliquot 100 µL into microfuge tubes (cannot take repeated freeze-thaw cycles)
Coat coverslip bottom dishes with either:
- 0.75 mL 0.1 mg/mL: 0.015 mL (5 mg/mL) + 0.735 mL PBS
- 0.75 mL 0.5 mg/mL: 0.075 mL (5 mg/mL) + 0.675 mL PBS
Incubate the coverslip dishes for 1 hour 37˚C
Remove the solution
rinse in PBS
Air dry the dish.
Cyclin InhibitorCyclin Inhibitor kdorfman Wed, 04/25/2018 - 19:55
CKD1 inhibitor IV RO3306
1 mM in DMSO
A cyclin-dependent kinase inhibitor protein inhibits cyclin-dependent kinase. Several function as tumor suppressor proteins. Cell cycle progression is negatively controlled by cyclin-dependent kinases inhibitors (called CDIs, CKIs or CDKIs). CDIs are involved in cell cycle arrest at the G1 phase.
CyclohexiamideCyclohexiamide bcrcstaff Thu, 03/21/2019 - 13:28
Protein synthesis inhibitor
MW = 281.35 g/mol
Cytochalasin DCytochalasin D kdorfman Mon, 04/23/2018 - 18:21
MW = 507.62
Sigma C2618: 200 µL 5 mg/mL (~10 mM) in DMSO
Sigma C8273: 5 mg. Dissolve in 1 mL DMSO
- alkaloid mycotoxin produced by Helminthosporium and other molds.
- disrupts actin microfilaments
- binds to F-actin polymer
- prevents polymerization of actin monomers
- Aliquot 10 µL 10 mM
- Add 90 uL DMSO (makes 1 mM)
- Dilute this 1:1000 into medium (makes 1uM)
Dilute the 1 uM to desired working concentration
Store in the dark, in the freezer
Adding 490 µL of non-CO2 medium makes 500 µL of 200 µM
Final dilution should contain no more than 0.1% DMSO
250 nM in the dish should give an effect
15 - 30 min
FibronectinFibronectin kdorfman Thu, 04/26/2018 - 17:15
GSA 10GSA 10 kdorfman Thu, 01/28/2021 - 18:39
Smo (Smoothened) receptor* agonist
Tocris 4918 10 mg
MW = 459.94
Make a 5 mM stock solution in 4.34 mL DMSO (heat to 50C to dissolve)
*Non-classical G-protein-coupled receptors that belong to the Frizzled family. Smoothened receptors lack the ability to directly interact with their endogenous ligand, Hedgehog (Hh). In the resting state, Smo receptors are bound to patched (Ptc).
Does not recognize the classic cyclopamine (Cat. No. 1623) binding site. Does not promote Smo translocation to the primary cilium; is strongly potentiated by forskolin and cholera toxin. Displays anti-adipogenic effects in vitro, mediated by a non-canonical Hedgehog signaling pathway. Promotes differentiation of multipotent mesenchymal progenitor cells into osteoblasts.
H-89H-89 kdorfman Mon, 03/22/2021 - 18:08
H 89 hydrochloride Tocris 2910
1 mg/mL in DMSO
HU (Hydroxyurea)HU (Hydroxyurea) kdorfman Wed, 01/06/2021 - 18:29
|HU||0.2 M||76||Koshand lab 2M in H2O|
Hydroxyurea: (Sigma H8267-1g)
- To make 50 mL YPD with 0.2M HU
vi = (0.2M * 50 mL)/2M = 5 mL
100µL 2M stock per mL medium
Buy 1 g, make 6.6 mL 2M stock
- MW = 76, so 1 g = 1/76 mol = 0.0132 mol
- vol = (1000 mL/2 mol) * 0.0132 mol = 6.6 mL
See yeast recipes for QBoC https://wahoo.cns.umass.edu/node/554/
HydrocorisoneHydrocorisone kdorfman Mon, 03/22/2021 - 18:06
Fisher A16292 (Alfa Aesar) 1 g
1 mg/mL in 100% ethanol.
ImportazoleImportazole kdorfman Thu, 05/10/2018 - 14:25
Importazole is an inhibitor of importin-β transport receptors.
During interphase, the transport receptor importin-β carries cargoes into the nucleus, where RanGTP releases them. Importazole somehow disrupts the importin/RAN interaction. Importazole is selective among other transporters. Compounds are imported into, but not out of, cells.
A cell-permeable diaminoquinazoline compound that selectively blocks importin-β-mediated nuclear import of NLS bearing cargos in a reversible manner.
Soluble in DMSO (25 mg/ml; clear, colorless solution)
5 mg/mL ailquots in freezer
IonomycinIonomycin kdorfman Mon, 04/23/2018 - 18:47
Raises intracellular calcium levels
Fisher or Invitrogen (Life Technologies) I24222
1 mM in DMSO or EtOH. 1 mg makes 1.34 mL stock.
3 µM Ionomycin in HBS
Dilute stock 1:333 in HBS: 3 µL/1 mL
24 µL in 8 mL, aliquot 800 µL
They only need 750µL, because they do one experiment in HBS, then two in Ca-free HBS.
3 µM Ionomycin in Ca-free HBS
Dilute stock 1:333 in Ca-free HBS: 3 µL/1 mL
45 µL in 15 mL
Takes a long time to thaw. Vortex.
Aliquot 1.55 mL so they can do two 750 µL experiments
JasplakinolideJasplakinolide kdorfman Thu, 04/26/2018 - 17:44
(out of stock 2018)
- Jasplakinolide – Santa Cruz biochemical, product sc202191 F
- inducer of actin polymerization & stabilization; inhibits actin filament disassembly
- F-actin probe
- MW = ~710
- powder is stable frozen over a year
- stock is stable 3-4 mo at -20C
- Make 1 mM stock with 50 µg in 70 µL DMSO
- Add 5 mL non-CO2 medium to make ~14 µM
- 50 nM to 5 μM working range
- minutes to hours incubation time
LDLLDL kdorfman Fri, 05/11/2018 - 16:29
Low-density lipoprotein from human plasma, Dil complex (Dil LDL)
DO NOT FREEZE
Life Technologies L3482
LamininLaminin kdorfman Thu, 04/26/2018 - 18:16
Laminin from mouse BD354232
1 mL 1 mM
LatrunculinLatrunculin kdorfman Thu, 04/26/2018 - 17:46
Alexis Biochemicals 350-036-C100 Catalog # t110
Inhibits actin polymerization and disrupts microfilament organization, as well as microfilament-mediated processes
Reported to be 10 to 100-fold more potent than the cytochalasins. May act more slowly, though. Lots of variation between cell lines.
Inactivated by FBS. (Rinse medium off before treatment.)
MW = 395.5
Soluble in DMSO and ethanol.
Store in freezer.
Active concentration range: 90 nm ( =~0.1µM) to 2.5 µM
(2.5 µM = 25 x 100 nm)
Stock is 1mm
|µL 1mM stock||µL serum-free buffer or medium||µM final concentration|
*Use this to make further dilutions
ML-7ML-7 kdorfman Wed, 04/25/2018 - 19:42
ML-7 Sigma, 12764.
selective myosin light chain kinase inhibitor
- soluble 10mg/mL in 50% EtOH
- MW = 452.74
- 5 mg in bottle
- add 1100 µL to make 10 mM solution
- used at ~50 µM (1 µL/1mL) (Pat says 75 µM)
- 75 µL 10 mM + 5 mL non-CO2 medium makes 150 µM
- make 15 µL aliquots of 10 mM. Add 985 µL medium to make 150 µM
Melanocyte Stimulating HormoneMelanocyte Stimulating Hormone kdorfman Wed, 04/25/2018 - 20:48
Sigma M4135 1 mg
Molecular Weight 1664.88
From Sigma: Amino Acid Sequence:
Pituitary hormone which causes darkening skin pigmentation from amphibians to humans. In mammals, it can also have behavioral effects on learning, attention, and memory.
Hormone that stimulates melanogenesis; facilitates learning and memory; affects inflammatory and immune responses and peripheral nerve regeneration.
α-Melanocyte-stimulating hormone (α-MSH) acts as an anti-inflammatory agent via down regulating the production and activity of the pro-inflammatory cytokines interleukin-1 (IL-1), tumor necrosis factor (TNF)-α and IL-6 expressed in various cells of the immune system. It also controls the nitric oxide production associated with inflammation. α−MSH inhibits nuclear factor-κB (NF-κB)-dependent gene transcription and NF-κB pathway induced by TNF and other inflammatory agents. This activity of α-MSH is mediated through the production of cyclic adenosine monophosphate (cAMP) and activation of protein kinase A (PKA) enzyme. α–MSH functions as a potent therapeutics for various conditions resulted through NF-κB activation including, inflammatory diseases, human immunodeficiency virus (HIV) replication in AIDS (acquired immunodeficiency syndrome), and septic shock. α-MSH has an essential role to play in melanin production in animals. α-MSH regulates development of several skin diseases, including cutaneous inflammation and hyper-proliferative skin diseases. Linkage
Derived from ACTH 1-13 Other Notes
Lyophilized from 0.1% TFA in H2O Packaging
1, 5 mg in glass bottle Application
α-Melanocyte stimulating hormone (α–MSH) has been used for following studies: • it has been Intracerebroventricularly (icv) injected to mice for behavioral studies. • to determine the effect of α−MSH on growth of stationary Nb 2 node lymphoma cell cultures. • to determine the effect of α−MSH on leptin secretion in the primary cultures of differentiated adipocytes. • α−MSH promoted melanin production in the B16-F1 cells from murine melanoma cell line. General description
α-Melanocyte-stimulating hormone (α-MSH) is a tridecapeptide, mostly produced by the cells in the brain, pituitary and circulation. Pro-inflammatory cytokines or UV light induced epidermal cells such as keratinocytes and melanocytes synthesize and discharge α–MSH. Poopiomelanocortin (POMC) acts as a precursor for α-Melanocyte-stimulating hormone (α-MSH) production.
MonastrolMonastrol kdorfman Thu, 05/10/2018 - 15:17
Inhibits kinesin 5
200 mM in DMSO
Final concentration 200 uM
5.5 uL aliquots
MonensinMonensin kdorfman Wed, 04/25/2018 - 21:25
Monensin sodium salt
soluble in methanol 50 mg/mL
insoluble in water
soluble in DMSO, ethanol
ionophore which disrupts the structure of the Golgi apparatus and inhibits vesicular transport in eukaryotic cells
inhibits transition from G1 to S
antiprotozoal, antibacterial, or antifungal agent
1 µM = half-maximal inhibitory effect
Nerve Growth FactorNerve Growth Factor kdorfman Fri, 05/11/2018 - 16:16
Nerve Growth Factor 2.5S
Harlan.com Cat no. 5025
From Sigma: 2.5S subunit of nerve growth factor-7S (NGF-7S) is essentially the β-subunit when isolated from male mouse submaxillary glands under initially dissociative conditions by a modification of the method of Bocchini and Angeletti.
Stored at 4C
NocodazoleNocodazole kdorfman Mon, 04/23/2018 - 17:51
Prevents polymerization of microtubules. Prevents cells from entering metaphase.
*Acros 358240100, 10 mg
*Soluble in DMSO
*Dilute stock in medium to treat cells.
*Make a 10 mg/mL (=33mM) stock: Add 1 mL DMSO to 10 mg in bottle.
- Aliquot 3 µL 33 mM per tube.
*Working concentration range is usually 100nM - 30 µM.
add 97 µL DMSO to tube to make 100 µL of 1 mM
33 µL 1mM + 10 mL medium = 3.3 µM
PMSFPMSF bcrcstaff Thu, 03/21/2019 - 14:14
Serine protease inhibitor
Plant hormonesPlant hormones bcrcstaff Thu, 03/21/2019 - 14:49
|ABA Plant hormone involved in growth and stress response||powder||362A||fridge||50 mM in EtOH||Fisher AC133480010|
|ABA||powder soluble 20 mg/mL in DMSO, ethanol||362A||freezer||PlantMedia 30631017-1
soluble in methanol
|Ascorbic acid||Enzyme cofactor||Powder||362A||RT||Fisher S26184|
|Caffeine||Growth inhibitor||Powder||362A||RT||Fisher ICN15011483|
|Chitosan medium viscosity||Component of fungal cell walls and arthropod exoskeletons||Powder||362A||RT||Bio-World 40300161-1|
|Cycloheximide||Protein synthesis inhibitor||Powder||362A||RT||Fisher AC357420010|
|Gibberellic acid||breaks seed dormancy||13 mg/mL (=37.5mM) solution||362A||4C||PlantMedia 30631025-1|
|IAA||auxin growth regulator
stimulates root growth
|solid||362A||4C||100 mM in EtOH||PlantMedia 30631010-|
|Imidacloprid (pestanal)||Systemic insecticide||neat||362A||dessicator||Sigma 37894|
|kinetin||Plant cytokinin||Powder||362A||RT||Sigma K0753|
|Lactic acid||Plant growth stimulator||69% soln aq||362A||dessicator||Fisher AC250300100|
|Methyl jasmonate||Pathogen response||Liq||362A||RT||PlantMedia30631015-3|
|Nicotine||Plant defense chemical||liq||362A||dessicator||Fisher AC181420050|
|Salicylic acid||Response to abiotic stress||Powder||362A||RT||100 mM in EtOH||Fisher S25515|
Poly-L-LysinePoly-L-Lysine kdorfman Thu, 04/26/2018 - 17:04
Non-specific attachment factor for cells; use it to coat coverslips.
- Add 50 ml of sterile tissue culture grade water to 5 mg of poly-lysine.
- Aseptically coat culture surface with 1 ml per 25 cm2 of solution. Rock gently to ensure even coating of the culture surface.
- After 5 minutes, remove solution by aspiration and thoroughly rinse surface with sterile tissue culture grade water.
- Allow to dry at least two hours before introducing cells and medium. If glassware or slides must be sterilized after coating with poly-lysine, γ-irradiation is recommended instead of autoclaving.
Can also buy poly-lysine coated slides: Fisher 6776215
PurmorphaminePurmorphamine kdorfman Thu, 01/28/2021 - 18:25
Tocris 4551 10 mg
MW = 525.12
Make a 10 mM stock in DMSO in 1.9 mL DMSO
STLCSTLC kdorfman Mon, 04/23/2018 - 17:51
- STLC Sigma 164739 (Fisher 50-703-1833), MW = 363.47
(+)-S-Trityl-L-cysteine is a cell-permeable selective inhibitor of mitotic kinesin Eg5 and ATPase activities.
KIF11 (also known as kinesin-5 and Eg5) is a homotetramer which cross-links anti-parallel microtubules in the mitotic spindle to maintain spindle bipolarity. The motor domain or motor head is at the N-terminus and performs ATP hydrolysis and binds to microtubules. (https://en.wikipedia.org/wiki/Kinesin_family_member_11)
Make 100 mM stock:
- 1 g in bottle.
- Mix with 27.5 mL DMSO
- heat to 65C (~10 min), vortex
- if necessary, filter sterilize to remove insoluble particles.
- save as 1 mL aliquots in freezer. (dilute 1:10,000 to use) (dilute 1:100 to make 1 mM)
Make 1 mM stock from the 100 mM stock
- 10 µL 1mM stock
- 990 µL DMSO
- it will crystallize in the refrigerator. Warm and vortex to redissolve it.
Make 10 µL aliquots of 1 mM stock. Freeze
- label says to add 990 µL medium to make 10 µM working solution
TaxolTaxol kdorfman Mon, 04/23/2018 - 17:51
Taxol (20 µM final)
Fisher 109710 Paclitaxel.
Sigma T7402 1mg
MW = 853.91
- Stabilizes the microtubule polymer
- Protects microtulues from disassembly.
- Prevents metaphase spindle configuration
Blocks progression of mitosis and prolonged activation of the mitotic checkpoint triggers apoptosis or reversion to the G-phase of the cell cycle without cell division.
soluble in DMSO, not water.
Add 0.117 mL DMSO to make a 0.01M (=10 mM) stock solution from the 1 mg powder
Aliquot 10 µL 10 mM (if it precipitates, add 10 uL DMSO and vortex, then add 480 uL medium or buffer)
- add 490 µL to make 200µM
- dilute into medium from there
Thyroid hormonesThyroid hormones kdorfman Fri, 09/06/2019 - 18:38
5 mL per well for treatment
PTU (inhibitor) Sigma p3755
- 50 mg/mL in 1M NaOH
- 16 mg/mL in alcohol
- 1 mg/mL in RT water
- 10 mg/mL in boiling water
In order to get the following doses, best to make it in hot fish water; otherwise, the NaOH or EtOH will be too concentrated in the final dilution.
dose 1: 0.5 mM
dose 2: 1.0mM
L-T4 (thyroxin) Sigma T2376
- 50 mg/ml in 4M NH4OH in MeOH
- 0.5 mg/mL in 1:5 DMSO:PBS
Solution is good for ~ 1 day.
Make a 1 mM stock in 1M NaOH. Takes a long time. Vortex.
Priya's recipe: 1 mg/mL in 1M NaOH (~25.7 uM stock)
dose 1: 100 nM (=0.1 uM)
dose 2: 300 nM (=0.3 uM)
Trichostatin ATrichostatin A kdorfman Wed, 04/25/2018 - 19:52
Histone deacetylase Inhibitor
Trichostatin A (TSA), an antifungal antibiotic produced by Streptomyces hygroscopicus, is a potent and specific inhibitor of histone deacetylases (HDACs), which are overexpressed in various cancers and closely correlate with oncogenic factors.
Trichostatin A is active at nanomolar concentrations in mammalian cells. By suppressing the activity of HDACs, it leads to increased histone acetylation, thereby causing highly acetylated histones to accumulate in the cell . This in turn induces enhanced expression of specific genes that elicit extensive cellular morphologic and metabolic changes such as growth arrest, differentiation and apoptosis. At submicromolar concentrations Trichostatin A has been shown to induce apoptosis in diverse cancer cells while exhibiting very low toxicity to normal cells.
TSA is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, TSA should be directly dissolved in 0.1 M HCl (1.3 mg/ml) and then neutralized with PBS (pH 7.2) to achieve the desired concentration or pH. Approximately 0.7 ml of PBS (pH 7.2) is required to neutralize 1 ml of the acidic solution. We do not recommend storing the aqueous solution for more than one day.
ValinomycinValinomycin kdorfman Wed, 04/25/2018 - 21:03
Potassium Ionophore, MW: 1111.32
Insoluble in water
stock is 10 mg/mL in DMSO
- cyclododecadepsi-peptide ionophore antibiotic.
- potassium ionophore that transports K+ across biological and artificial lipid membranes.
- can induce K conductivity in cell membranes
- uncouples oxidative phosphorylation,
- induces apoptosis in murine thymocytes, and in pre-B cell.
- inhibits NGF-induced neuronal differentiation
- antagonizes ET-induced vasoconstriction.
- Useful in studies of K transport in mitochondria.
VasopressinVasopressin kdorfman Mon, 04/23/2018 - 17:50
causes calcium oscillations
1 mM stock in water
(NO CALCIUM, so stock can be used for Ca-free solutions in Lab 7.2)
1 mg in bottle, MW = 1084
Add 0.92 mL to make 1mM stock
1 µL aliquots in freezer
1 µM dilution to make student solutions
1 µL 1 mM in 999 µL water
(NO CALCIUM, so stock can be used for Ca-free solutions in Lab 7.2)
By targetBy target kdorfman Wed, 06/20/2018 - 21:28
|Target or Effect||Drug(s)|
|Non-muscle myosin II ATPase||Blebbistatin|
|Internal calcium levels||Bradykinin|
|α-Mannose, α-Galactose sugars, glycoproteins and glycolipids||Concanavalin A|
|cyclin-dependent kinase||cyclin inhibitor|
|actin polymerization||cytochalasin D|