New drop down test

New drop down test kdorfman Tue, 10/02/2018 - 15:20

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mouse anti-LAMP1 (lysosomes)

GeneTex GTX13523

IgG

1 mg/mL

4C short term

Freeze 1 ┬ÁL aliquots

dilute 1:100 - 1:750 in 0.1% BSA in PBS

rinse in PBS

Fix in 4% paraformaldehyde 15 min for endosomes

in cold methanol for Golgi

0.5% saponin 5 min

1% BSA in PBS 30 min

1o Ab stain (0.1% BSA) overnight at 4C

wash 30 min in PBS

2o Ab 1 h 37C

wash 30 min PBS

mount in DAPI

reference