Goat anti-rat TRITC

From Sigma AP136R EMB Millipore

ab peak = 550nm; emission peak = 570 nm

Bottle contains 2 mg. Add 1 mL H2O + 1 mL glycerol to make 1 mg/mL

aliquot 2 µL or 5 µL antibody, freeze

add 198 µL or 495 µL PBS-Tw-BSA

use 50 µL per coverslip, diluted to 1/100 (Try 37C 30 min)