50X TAE stock

Buy from Fisher TAE 50x, pH 8.3, 4L, part #BP13324

from Maniotis

To make	mL 50X TAE
add:	g Tris base
add:	mL glacial acetic acid
add:	mL 0.5M EDTA
OR add:	g EDTA

pH to 8 with acetic acid or NaOH

should this be 8.4?

Ingredient	cf (mM)	1000	750	600	500	mL
Tris	2000	242	181.5	145.2	121	g
Acetic acid	1000	57.1	42.9	34.3	28.6	mL
EDTA 0.5 M	50	100	75	60	50	mL
OR
EDTA	50	18.62	13.965	11.172	9.31	g

pH to 8 with acetic acid or NaOH

Maniotis says to autoclave, but the salt concentration is so high that nothing will grow in it if you don't.