SC-complete-high-Ade

Liquid Medium

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Using 4X SC-AHLT (because 10x won't go into solution):

To make	mL SC-complete high ade liquid medium
Meanwhile, using	X SC-AHLT
Mix:	mL 10X SC–AHLT,
and :	mL 10X YNB+AS,
and:	mL 20% Glucose,
and:	mL 10x adenine hemi sulfate,
and:	mL 50X Tryptophan ,
and:	mL 100X Histidine .
and:	mL 10X Leucine

Bring to final volume with water, then filter sterilize.

Jeff Laney's original (discontinued - because you can't make 10X SC-AHLT) recipe:

Component	Volume (mL)
10X SC–AHLT	25
10X YNB+AS	25
10X SC–AHLT	25
20% Glucose	25
10x adenine hemi sulfate	25
50X Tryptophan	5
100X Histidine	2.5
10X Leucine	25
distilled water	to final volume of 250

Filter sterilize

Store at room temperature