SC -Leu High Ade 4X

Using dilution factor 4:
(Because 10x won't go into solution)

But the agar is so viscous that bubbles don't reach the surface. Hard to pour.

To make	SC-leu high ade agar plates
with	mm plate diameter
pour	mL per plate
start with:	mL water
add:	g agar, leave stir bar in
autoclave for:	minutes
Meanwhile, using	X SC-AHLT
Asceptically mix together, warm up, then add to autoclaved agar:	mL SC-AHLT,
and :	mL 10x YNB+AS ,
and:	mL 20% glucose ,
and:	mL 10X Adenine hemi sulfate,
and:	mL 50X Tryptophan,
and:	mL 100X Histidine HCl.
for a final volume of	mL

Mark with one green line