SC -Leu-low-Ade 4X

Using 4X SC-AHLT because 10x won't go into solution:

(BUT: Agar is too viscous - bubbles won't rise to the surface before it sets up.)

To make	SC-leu low ade agar plates
with	mm plate diameter
pour	mL per plate
start with:	mL water
add:	g agar, leave stir bar in
autoclave for:	minutes
Meanwhile, using	X SC-AHLT
Asceptically mix together, warm up, then add to autoclaved agar:	mL SC-AHLT,
and :	mL 10X YNB+AS,
and:	mL 20% Glucose,
and:	mL 10x adenine hemi sulfate,
and:	mL 50X Tryptophan
and:	mL 100X Histidine .
for a final volume of	mL

Mark with one green and one blue line.