Laney's original
Laney's original kdorfman Sun, 01/26/2020 - 17:02SC-Leu low-Ade plates
From Jeff Laney's recipe:
(BUT, can't make 10X SC-AHLT)
Per liter of medium, mix the following asceptically, and pre heat:
| Component | Volume (mL) |
|---|---|
| 10X SC-AHLT | 100 |
| 10X YNB+AS | 100 |
| 10X 20% glucose | 100 |
| 10X Adenine hemi sulfate | 2.5 |
| 50X Tryptophan | 20 |
| 100X Histidine HCl | 10 |
Add to 20g agar in 667.5 mL water, autoclaved 30 minutes
Mark with one green and one blue line.