SC -Leu-low-Ade 4X + powder

SC -Leu low Ade 4x + powder

Use the 4x SC-AHLT to mix dry ingredients

(4x already made; not enough dry left to start over.)

per Liter:

Agar 20 g + 617.5 mL water. Autoclave

Meanwhile, make:

Component	Volume (mL)
4X SC-AHLT	250
10X Adenine hemi sulfate	2.5
10X 20% glucose	100
50X Tryptophan	20
100X Histidine HCl	10

Add dry:

Component	g
YNB	1.7
Ammonium Sulfate	5

Filter sterilize, warm up, then add to molten agar

Mark with one green and one blue line