Addgene plasmid selection

When cells are medium-light, replace medium with 0.5 g/L G418 (= 0.5 mg/mL)

Next day, replace with medium with 1 g/L (=1 mg/mL)

Next day, 2 g/L (=2 mg/mL)

Solution is 50 mg/mL

Dilute 10 uL per mL to get 0.5 mg/mL

Dilute 20 uL per mL to get 1 mg/mL

Dilute 40 uL/mL to get 2 mg/mL