8X SC-HLT hi Ade concentrate

8X SC-HLT hi Ade concentrate kdorfman Fri, 01/22/2021 - 22:01

Concentrate to make SC-HLT High Ade agar

add 125 mL heated concentrate to autoclaved 875 mL water + 20 g agar right out of the autoclave and still hot

to make enough concentrate for:	Liter(s) SC-HLT high Ade agar medium
start with	mL water
add:	g YNB
and:	g ammonium sulfate
and:	g SC -HLT
autoclave for:	minutes (to get it into solution)
while it's still hot, add:	g glucose
and:	g Adenine hemi sulfate
and:	g tryptophane
and:	mL his 100X concentrate.
Bring to a final volume of:	mL 8X SC-L high Ade and filter sterilize