2021 DNA Extraction

2021 DNA Extraction kdorfman Fri, 02/19/2021 - 16:57

Equipment:

  • Grinder
  • tongs
  • Ice buckets
  • Centrifuge
  • Freezer boxes
  • Compost buckets
  • B-mercaptoethanol waste bucket in fume hood

Reagents:

  • 1 mL DEB per sample
  • 415 uL KOAc per sample
  • Fresh round bottom 2 mL tube with 1 mL 70% ethanol
  • 500 uL 95% ethanol
  • 50 uL TE per sample