2021 RNA extraction
2021 RNA extraction kdorfman Fri, 04/16/2021 - 13:50RNEasy Kit Qiagen 74904
Per reaction (6 x 23=138), :
- LN2
- Tissue flash frozen in LN2 in round bottom 2 mL tube with 2 grinding beads
- Buffer RLT 450 uL
- lilac QIAshredder in 2 mL collection tube
- pink tube
- 225 uL 100% ethanol
- 350 Buffer RW1 x 2 (see below)
- 10 uL DNAse (in RDD) IF NO INTRON
- 70 uL RDD
- 350 uL RW1
- 500 uL RPE
- 500 uL RPE
- 30 uL RNAse free water
- 20 uL RNAse free water
| Reagent | uL per rxn | uL per 6.5 rxns |
|---|---|---|
| RLT | 450 | 2925 |
| EtOH | 225 | 1462.5 |
| RW1 | 700 | 4550 |
| DNAse | 10 | 65 (have ~570 uL, more on the way) |
| RDD | 70 | 455 |
| RPE | 1000 | 6500 |
| H2O | 50 | 325 |