Fixative stock: 37% Formaldehyde: small bottle under chemical hood in prep lab.
TritonX-100: 10% stock, in hood in prep lab
To make 10 mL of fixative:
To a 15 mL conical plastic tube add: 1mL of 37% formaldehyde 0.5 mL of 10% triton 1 mL of 10x PBS dH2O to 10 ml mark (8.5 mL)
Make in fume hood
To fix the cells:
Remember to wear gloves and be very careful of the fixatives!!! Remember which side of the coverslip has the cells. Remember to discard used fix into the appropriate hazardous waste container in the chemical hood in the main lab.
Fixative Stocks: 32% Paraformaldehyde: bottle under chemical hood in main lab. Detergent: 10% triton X 100
To make 10 mls of fixative: Paraformaldehyde: 1 mL stock 10% triton: 0.5 ml 10X PBS -/- 1 mL add water make 10 mL (8.5 mL)
Use same method as Formaldehyde fixation.