M21 from stocks M21 from stocks kdorfman Wed, 12/15/2021 - 21:19 To make plates (100 mm)(for a final volume of mL of medium)start with: mL wateradd: mL 1M K2HPO4add: mL 1M KH2PO4add: g Difco Bacto Peptone, and bring to final volumeadd: g agarautoclave for: minutes Add 50 - 100 µg/mL ampicillin (carbenicillin) and 1 mM IPTG if needed