Naegleria 3
Naegleria 3 kdorfman Sat, 12/18/2021 - 12:00Cloning Bootcamp
- 37C heatblock
- 42C heatblock
- Age1
- 10X buffer NEB1.1
- rSAP
- Sterile water for cloning
- Ligase + 2X rapid lligase buffer
- 1 sample: PCR cleanup kit (Qiaquick) (one per group)
- 1 tube (5 rxns) Competent cells (DH5 alpha, just for cloning)
- 5x LB+Amp plates
- Sterile beads for spreading
- 30 ng/ul backbone plasmid for cutting (estimated 15n/ul after elution w/ 30ul)
- Gel purified inserts (gamma tub, centrin, sas-6 at 15ng/ul)
- LB (plain) for transformation recovery