Planting schedule 2022

Pots for students to plant seeds for genomic DNA

12 pots for Week 1 (1/26/22)

Planting for Week 5:

Arabidpsis essentials (2/23/22):

• 2 seeds in each of 6 rose pots every week,
• starting 1/12 (2 week before classes begin),
• for a total of 36 pots
• 6 1/2 MS plates 1 week before week 5 (seeds to fridge on 2/14/22)

Students plant mutant seeds:

• 12 groups, 2 pots mutants, 2 pots Col0
• for a total of 48 3" pots

Planting for Week 11:Gene expression planning:

Seeds on plates for RNA low and high iron experiments: ? at least 12 1/2 MS plates, 6 plates low iron.

5 seeds in each of 24 pots every week for 4 weeks, starting 6 weeks before week 11. (weeks 7, spring break, 8, and 9), for a total of 96 pots

1 row of seeds on each of 24 plates, 1 week before week 11

Date	plant	for
1/12	6 rose pots	6 week old Arabidopsis
1/19	6 rose pots	5 week old Arabidopsis
1/26	6 rose pots	4 week old Arabidopsis
2/02	6 rose pots	3 week old Arabidopsis
2/09	6 rose pots	2 week old Arabidopsis
2/16	6 1/2 MS plates	1 week old Arabidopsis
2/23
3/01	12 1/2 MS plates	seedlings on cellophane for cDNA (iron expt)
3/03	plates to growth chamber	start seedlings growing
3/07	24 pots	6 week old for gene expression
3/11	txfer plated seedlings	half to MS, half to low iron
3/14	harvest iron expt seedlings	Stavroula makes cDNA
3/14	24 pots	5 week old for gene expression
3/21	24 pots	4 week old for gene expression
3/28	24 pots	3 week old for gene expression
4/06	24 plates	2 week old for gene expression
4/13	24 plates	1 week old for gene expression