Sterilize seeds with EtOH & Tritonx

Sterilize seeds with EtOH & Tritonx kdorfman Mon, 02/14/2022 - 18:31
  • sterilize seeds in a solution of

    • 0.5 mL 70% EtOH,
    • 0.05% TritonX-100,
    • ~3 min mild agitation.
  • Let seeds settle, remove liquid

  • Replace with sterile water, 3X

  • Replace water with sterile 0.1% agar, 3X

  • Plate in a line, either

    • directly on agar, one seed per tiny drop or
    • on flattened cellophane (wet it if necessary)
  • Wrap plates with micropore tape

  • stack plates flat

  • cover in foil, refrigerate, 48 hours