Sterilize seeds with bleach

• Add ~1mL of bleach solution to each tube
• Cap and shake
• Leave tube on its side for 20-30 min, shaking periodically
• Put upright after last shake (so seeds settle)
• In laminar flow hood
  • Use a pipetman that has been sprayed with ethanol and air-dried in the hood
  • Remove as much bleach solution as possible from the tube
  • Add ~1 mL sterile ddH2O, mix and let seeds settle again
  • Do at least 4 washes, to dilute out the bleach
  • Add ~1mL sterile 0.1% agarose (so seeds will be suspended in the liquid)

Bleach solution for sterilizing seeds

• 7 mL sterile ddH2O (must be freshly made!)
• 3 mL Chlorox
• 1 uL Triton X-100 (detergent to help get bleach into seed crevices)

0.1% agarose for plating seeds

• 100 mL sterile ddH2O
• 0.1 g agarose . autoclave to melt; shake bottle after autoclaving