5.1 DNA extraction for genotyping

5.1 DNA extraction for genotyping kdorfman Tue, 03/22/2022 - 13:11

From Elizabeth Vierling

Per Reaction:

  • 500 uL Edwards Extraction Buffer (6.5 mL/pair, 100 mL/class)

  • 300 uL Isopropanol (3.9 mL/pair, 50 mL/class)

  • 100 uL T10E1 (1.3 mL/pair, 20 mL/class)

  • 1 2-mL grinding tube (12 tubes/pair, 50/class)

  • 2 steel beads (24 beads/pair, 300 beads/class)

Per class * Dresch ball mill grinder

  • 6 microcentrifuge

  • 6 vortex