Sybr Safe gel

Sybr Safe gel kdorfman Tue, 09/20/2022 - 15:01

1 uL /10 mL gel

gels:
%:
TAE: mL
agarose: g
SYBR: ┬ÁL

*Add weighed agarose to measured TAE in a flask (about half the maximum volume of the flask)

*Boil in the microwave CAREFULLY (power level 0.5) until completely dissolved (check by swirling) DO NOT LET IT BOIL OVER

*Allow to cool slightly

*Add SYBR-safe

*Aliquot into 50 mL conical tubes

*Put in rack in 65C waterbath

Clean-up

Everything can go in the trash or down the drain