EtBr gel

EtBr gel kdorfman Tue, 09/20/2022 - 15:02

Ethidium bromide gel

gels:
%:
TAE: mL
agarose: g
EtBr: ┬ÁL

*Add weighed agarose to measured TAE in a flask (about half the maximum volume of the flask)

*Boil in the microwave CAREFULLY (power level 0.5) until completely dissolved (check by swirling) DO NOT LET IT BOIL OVER

*Allow to cool slightly

*Add EtBr (in the fume hood)

*Aliquot into 50 mL conical tubes

*Put in rack in 65C waterbath

Clean-up

  • Rinse gel rigs within an inch of their lives

  • Put gels into zip lock bag, stick a hazardous waste sticker on it, schedule hazardous waste pickup (sign into CEMS)

  • pour used buffer into gallon jug with EtBr removing "tea bag", stir overnight, then pour down the drain