T7 RiboMAX

T7 RiboMAX kdorfman Wed, 09/21/2022 - 19:46

Transcription mix

Students make the transcription mix from the kit

1 reaction per group

Reagent Sample reaction
RiboMAX™ Express T7 2X Buffer 10 µL
linear DNA template (1µg total) 1– 8µL
Nuclease-Free Water 0–7µl
Enzyme Mix, T7 Express 2 µL
Final Volume 20 µL