Courses
Courses kdorfman Thu, 01/11/2024 - 18:27Courses taught in ISB
Bio 499CB (Loomis Cancer)
Bio 499CB (Loomis Cancer) kdorfman Thu, 09/08/2022 - 20:59Kari Loomis honors thesis seminar
Librarian does workshop on library research.
We do Bradford assay lab:
Explain Bradford assay.
Add some Bradford reagent to some BSA - Ask how much protein?
Need standards to compare to!
- Bradford concentrate from Bio-Rad 500-0006
- dilute 1:4 with water
- Make BSA solution 0.1 mg/mL (dilute from the test 2 m/mL test solution
- Students share a plate:
- one uses top row
- one uses bottom row
- write a program on PS2
Student protocol:
- Make a 2-fold dilution series of the BSA
- 0.5 mL water in each tube (except the first)
- 1 mL BSA into first tube
- .5 mL to next tube, mix, transfer to next, etc.
- Put 160 uL BSA into each well
- put 160 uL water in one well. (Why?)
- Add 40 uL Bradford
- Read plate
Data management
- Stupid Excel Tricks
- Art of the standard curve
- Make your own std curve.
- How would you estimate the concentration of protein in a solution that had an Abs of:
- 0.5
- 0.4
- 3
Marine Biology
Marine Biology kdorfman Mon, 01/15/2024 - 17:32Sea Water for 424
Sea Water for 424 kdorfman Mon, 01/15/2024 - 18:142 carboys SeaWater (31-35ppt salinity):
- 31-35g InstantOcean Reef Salt in 1 L
- Submerge a bubbler or a pump so that it is constantly being mixed
F2 Medium for algae (424)
F2 Medium for algae (424) kdorfman Mon, 01/15/2024 - 18:165 liters of f/2 medium for algae.
Re-inoculate once during week of 1/15
TA & Students will re-inoculate once monthly
50 mL/month x 4 months x 16 students = 3200 mL
Make 4 L for the semester.
Filter sterilize
Perfect Plant 2024
Perfect Plant 2024 kdorfman Wed, 01/10/2024 - 21:51Bio 427 - Madelaine Bartlett
Wednesdays
| Date | subject | prep |
|---|---|---|
| 2/7 | plant architecture | Find the leaf. lycophytes, bryophytes, diversity, alstoemeria, Ruscus |
| 2/14 | ferns and lycophytes | ???? clv1 and wus mutants? from maize, tomato, arabidopsis; moss phyllotaxy |
| 2/21 | seed plants | life cycles - moss gametophytes, sporophytes; tree thinking/traits on a tree. moss gametophytes, fern gametophytes, prepared slides |
| 2/28 | individual plant 1 | |
| 3/6 | fruit diversity | fruit diversity (grocery store) including Citrus diversity; fas PCR, fruit description |
| 3/13 | exam 1 | |
| 3/20 | spring break | |
| 3/27 | ABC model | arabidopsis and maize ABC(E) mutants |
| 4/3 | individual plant 2 | |
| 4/10 | floral diversity | grocery store floral diversity |
| 4/17 | exam 2 | |
| 4/24 | leaf development | patterning mutants - ask Aman, Annis |
| 5/1 | leaf diversity | Leaf morphological and anatomical diversity (C3 and C4), practice making hand sections |
| 5/8 | presentations |
2024/02/07
2024/02/07 kdorfman Thu, 01/11/2024 - 18:47Perfect Plant Lab 1
Topic: Major lineages of land plants, basics of plant architecture
Plants needed: NHC diversity; maize, arabidopsis, tomato
Prepared slides: Meristems
Materials:
- dissection tools
- hand section materials
- microscopes
2024/02/14
2024/02/14 kdorfman Thu, 01/11/2024 - 19:01Perfect Plant Lab 2
Topic: Fern lifecycle
Resources: C-fern Manual
Materials: dissection and hand section materials, microscopes
Plants: C-fern gametophytes; NHC diversity
Prep: Sow Arabidopsis ABC mutants
2024/02/21
2024/02/21 kdorfman Thu, 01/11/2024 - 19:06Perfect Plant Lab 3
Topic: Seed plants
Plants needed: meristem mutants; NHC diversity
Prepared Slides: gametophytes; embryos
Materials: dissection and hand section materials, microscopes; maybe stuff for pollen tube growth
2024/02/28
2024/02/28 kdorfman Tue, 01/16/2024 - 21:37Individual Plant Day 1