Fern Culture Media
Fern Culture Media kdorfman Thu, 01/11/2024 - 19:16Pre-made fern medium, Carolina 156780, sold in 60 mL or 400 mL
Carolina recommends 10 mL per plate. I use 9 mL
To get gametophytes in all stages, sow fern spores in 12 60 mm dishes 4 times:
- 1/15
- 1/22
- 1/29
- 2/2 or Friday 2/3
Fern Medium from scratch
Fern Medium from scratch kdorfman Fri, 01/12/2024 - 21:18To make one liter
800 mL water
10X macronutrients: 100mL
200X micronutrients: 5 mL
100X Chelated Iron solution: 10 mL
pH to 6 with NaOH
Bring final volume to 1 L
Add 10 g Bacto agar (others may not work)
Autoclave 15 min
10X fern macronutrients
10X fern macronutrients kdorfman Fri, 01/12/2024 - 21:24200X fern micronutrients
200X fern micronutrients kdorfman Fri, 01/12/2024 - 21:26Fern medium from powder
Fern medium from powder kdorfman Wed, 01/24/2024 - 14:20Mix contents of vial with 800 mL water
pH to 6 (NaOH)
Bring to 1 L
For single batch:
Add 10g Bacto Agar
Autoclave 45 min
For small batches:
Aliquot 167 mL into 6 200 mL media bottles
Add 1.67 g Bacto Agar to each bottle
Autoclave 30 min