HeLa for 2nd day G&GA HeLa for 2nd day G&GA kdorfman Fri, 08/23/2024 - 16:47 9 HeLa (Vikash) coverslips Fixed in paraglut Stained for phospho H3 rabbit anti PHH3 (1:2000) goat anti-rabbit FITC (1:80) Stained for alpha tubulin: rat anti tubulin (directions on tube) goat anti rat TRITC (1:1000) Mounted in DAPI Note: Red tubulin was gorgeous! Green PHH3 not so much