Supplies
Supplies kdorfman Thu, 11/03/2011 - 19:35Get coverslip-bottom dishes from Cellvis, much cheaper than MatTek.
Celltreat also has them, about $2/dish:
https://www.celltreat.com/30mm-x-10mm-tissue-culture-treated-dish-15mm-…
Microscope bulbs for phase: 12V 100 W
- Philips 77241 or
- Osram HLX 64623
http://www.planetbulb.com/osram-eva-64623-hlx-54251/
Microscope bulbs for Zeiss:
- 38-01-77 6V 15W
- OQ-77Z 3800-18-1740 6V 15W or
- WW-6W51-8 from interlight.biz
Microscope bulbs for Olympus: BLC 120V 30W S11 BA15D from interlight.biz
Nucleofection kit
Ingenio Electroporation kit, MIR50112
mounting medium (SouthernBiotech Dapi Fluoromount-G, Fisher OB010020)
Hoechst solution Invitrogen™ Hoechst 33342, Trihydrochloride, Trihydrate - 10 mg/mL Solution in Water Nucleic acid stain Invitrogen™ H3570 cheaper than Thermo Fisher