Supplies
Supplies kdorfman Thu, 11/03/2011 - 19:35Get coverslip-bottom dishes from Cellvis, much cheaper than MatTek.
Celltreat also has them, about $2/dish:
https://www.celltreat.com/30mm-x-10mm-tissue-culture-treated-dish-15mm-…
Microscope bulbs for phase: 12V 100 W
- Philips 77241 or
- Osram HLX 64623
http://www.planetbulb.com/osram-eva-64623-hlx-54251/
Microscope bulbs for Zeiss:
- 38-01-77 6V 15W
- OQ-77Z 3800-18-1740 6V 15W or
- WW-6W51-8 from interlight.biz
Microscope bulbs for Olympus: BLC 120V 30W S11 BA15D from interlight.biz
Nucleofection kit
Ingenio Electroporation kit, MIR50112
mounting medium (SouthernBiotech Dapi Fluoromount-G, Fisher OB010020)
Hoechst solution Invitrogen™ Hoechst 33342, Trihydrochloride, Trihydrate - 10 mg/mL Solution in Water Nucleic acid stain Invitrogen™ H3570 cheaper than Thermo Fisher
Immersion Oil
For upright microscopes: Low viscosity
For inverted: high viscosity
Roy Kinoshita (MVI) says to use Type B oil ($10.00 for 1oz) for the fluorescence scopes.
Nikon recommends Type B for their Perfect Focus System (PFS), which maintains focus continuously on the inverted, almost always in fluorescence, and designed for long-term imaging. If the oil dries up or seeps down from low viscosity, PFS would fail after a few hours of imaging and people would not be happy. I believe the viscosity is 1250 cs.
From Cargille:
| type | viscosity | RI @ 546 | background fluorescence |
|---|---|---|---|
| A | 150 | 1.518 | low |
| FF | 170 | 1.4811 | virtually zero |
| LDF | 500 | 1.518 | very very low (for high res fluorescence) |
| HF | 700 | 1.518 | very very low |
| 37LDF | 1250 | 1.5181 | low (for use only at 37C) |
| B | 1250 | 2100 | low |
| NVH | 21000 | 1.5180 | low (larger coverslip-to-lens distance) |
| OVH | 46000 | 1.5178 | low |