Pipettes and tips

Pipettes and tips kdorfman Thu, 06/18/2009 - 16:04

Classroom pipettes

Classroom pipettes kdorfman Thu, 01/03/2013 - 17:41

The pipettes in use in the ISB are Rainin micropipettors :

  • Pipet-Lite® with LTS®, which takes LTS cylindrical tips, and has red, green, or blue labels on the plunger

LTS PIPETTES (rooms 260, 262A, 268, 360, 364, 366A, 368)

TIPS

Plunger max uL part #
Blue 1000 Rainin GPS-LTS 1000uL tips (30389292), in GPR-L1000 (blue) racks
Green 200 Rainin GPS-LTS 250 uL tips (30389299), in GPR-L250 (green) racks
Red 20 Rainin GPS-LTS 20uL tips (30389291), in GPR-L10 (red) racks

  • A 1-12 (264)
  • B 1-12 (368)
  • C 1-12 (364)
  • D 1-11 (360)
  • E 1-5 (371)

Colorimetric Pipetting Exercise

Colorimetric Pipetting Exercise kdorfman Wed, 06/21/2023 - 18:16

Mix 200 mM mono- (acid) and di-basic sodium phosphate (base) with bromothymol blue to get a range of colors from yellow to blue.

Put a 5 uL drop of bromothymol blue in each square, then add the given amounts of sodium phosphate

pH 6.0 6.2 6.4 6.6 6.8 7.0 7.2 7.4 7.6 7.8
Na2HPO4 2.5 3.7 5.3 7.5 9.8 12.2 14.4 16.2 17.4 18.3
NaH2PO4 17.5 16.3 14.7 12.5 10.2 7.8 5.6 3.8 2.6 1.7

Per exercise, need:

  • 107.3 uL Na2HPO4
  • 92.7 uL NaH2PO4
  • 50 uL bromothymol blue (0.04% aqueous) (e.g., Fisher LC120501, $25 for 500 mL))
  • printed buffer table (see linked file)
  • parafilm

So aliquot

reagent actual vol aliquot
Na2HPO4 107.3 250
NaH2PO4 92.7 250
bromothymol blue 50 125

Eppendorf Repeater

Eppendorf Repeater kdorfman Mon, 09/04/2023 - 20:17

Use Combitips Advanced:

Dial # aliquots 0.1 mL 0.2 mL 0.5 mL 1.0 mL 2.5 mL 5.0 mL 10 mL 25 mL 50 mL
white lt blue purple yellow green blue orange red gray
µL µL µL µL µL µL mL mL mL
* 100 1 2 5 10 25 50 0.1 0.25 0.5
1 50 2 4 10 20 50 100 0.2 0.50 1
* 33 3 6 15 30 75 150 0.3 0.75 1.5
2 25 4 8 20 40 100 200 0.4 1.00 2
* 20 5 10 25 50 125 250 0.5 1.25 2.5
3 16 6 12 30 60 150 300 0.6 1.50 3
* 14 7 14 35 70 175 350 0.7 1.75 3.5
4 12 8 16 40 80 200 400 0.8 2.00 4
* 11 9 18 45 90 225 450 0.9 2.25 4.5
5 10 10 20 50 100 250 500 1 2.50 5
* 9 11 22 55 110 275 550 1.1 2.75 5.5
6 8 12 24 60 120 300 600 1.2 3.00 6
* 7 13 26 65 130 325 650 1.3 3.25 6.5
7 7 14 28 70 140 350 700 1.4 3.50 7
* 6 15 30 75 150 375 750 1.5 3.75 7.5
8 6 16 32 80 160 400 800 1.6 4.00 8
* 5 17 34 85 170 425 850 1.7 4.25 8.5
9 5 18 36 90 180 450 900 1.8 4.50 9
* 5 19 38 95 190 475 950 1.9 4.75 9.5
10 5 20 40 100 200 500 1000 2 5.00 10

Prep Room Pipettes

Prep Room Pipettes kdorfman Thu, 01/03/2013 - 18:51

Rainin

Shop Rainin

F1 (362A)

  • 10 mL
  • 5 mL
  • 1 mL
  • 200 uL
  • 20 uL
  • 10 uL

F2 ( 266A)

  • 5 mL
  • 1 mL
  • 200 uL
  • 20 uL
  • 10 uL

F3 (donated to Animal Science)

5 mL pipette SL-5000XLS
Tips: LTS 5 mL 192/8 RT-L5000
Item # 17002937
Rainin Hinged Racks Box reads 30389256

10 mL pipette
Tips LTS 10 mL Prstrl 75/Pkg RC-L10MLS pre-sterilized, individually wrapped
Item #17005940


Eppendorf Repeater Plus in 362

Combitips Advanced

Get prices on tips from Krackeler