3.2: Immunofluor
3.2: Immunofluor kdorfman Wed, 11/23/2011 - 16:55Immunofluorescence Labeling
Turn on the Incubator
Reagents
PBS (warm) for rinsing medium off cells before fixation
PBS-Tw-Az for rinsing coverslips (in carboy)
2% BSA in PBS to make antibodies
Fixative
- paraglut for students
- methanol in freezer for certain antibodies
Slides
Nail polish
Mystery Antibodies for 2012
No. | Antibody | from | fixative | secondary | filter cube |
---|---|---|---|---|---|
1 | Golgi | KD | formaldehyde | goat anti mouse | G |
2 | LAP2 | PW | methanol | goat anti mouse | G |
3 | gamma tub | PW | paraglut or form | goat anti rabbit | G |
4 | ZO1 | KD | methanol or paraform | goat anti rabbit | G |
5 | vinculin | PW | formaldehyde | goat anti mouse | G |
Cells for 3.2
Cells for 3.2 kdorfman Tue, 12/06/2011 - 15:01Immunofluorescence Labeling