MS complete

MS complete kdorfman Mon, 12/19/2011 - 20:45
ingredient 750 800 1000 1200 1600 mL
number of plates 25 27 33 40 53
ddH2O* 450 480 600 720 960 mL
10X macronutrients 1 75 80 100 120 160 mL
10X micronutrients 2 75 80 100 120 160 mL
MES 0.375 0.4 0.5 0.6 0.8 g

*Add the other salt mixtures to the water to prevent precipitation

pH to 5.7 with 1M KOH (initial pH = ~3.66) (needs ~720 µL/L)

Bring to volume with distilled water

ingredient 750 800 1000 1200 1600 mL
bacto or phyto agar 7.5 8 10 12 16 g

autoclave with stir bar in flask or bottle

Stir until cool enough to handle

Pour 30 mL per plate (use the deep ones)

(Or Sigma M5524 - macro and micro nutrients together.)

  1. Sigma M 0654 Murashige and Skoog basal salt macronutrient solution (Krackeler 45-M0654-1L-EA) ~$26 ↩︎

  2. Sigma M 0529 Murashige and Skoog basal salt micronutrient solution (Krackeler 45-M0529-1L-EA) ~$26 ↩︎

MS nutrients

MS nutrients kdorfman Mon, 02/13/2012 - 19:56

MS macronutrients 10X

Sigma M 0654

Component mg/L
Potassium phosphate monobasic 170
Magnesium sulfate 180.7
Calcium chloride anhydrous 332.2
Ammonium nitrate 1650
Potassium nitrate 1900

MS micronutrients 10X

Sigma M 0529

Component mg/L
Boric acid 6.2
Cobalt chloride . 6H2O 0.025
Cupric sulfate. 5H2O 0.025
Ferrous sulfate.7H2O 27.8
Manganese sulfate. H2O 16.9
Molybdic acid (sodium salt) o 2H2O 0.25
Na2-EDTA 37.3
Potassium iodide 0.83
Zinc sulfate.7H2O 8.6