Resistance 1

1/31/12 and 2/2/12

• 6 tubes of freshly growing E. coli BZB1011 in LB (one on each set of desks) from Peg's lab
• 6 tubes with 20 mL sterile LB (one on each set of desks)
• ~150 Sterile swabs (one beaker full per set of desks) (= 2 100-packs, sterilized)
• 12 Pipetteman and tips (1 per 2 students) (for 10 to 100 µL volumes)
• ~125 MacConkey plates (about 4 per student with extras available) @ 25 mL/plate = 3125 mL (@ 50 g/L = 62.5 g)
• 24 black markers
• shelf in an incubator
• shelf in a refrigerator
• camera to photograph plates (or students use iphones)
• scale for weighing 25 grams
• weigh boats
• vortexes

Have on hand for student use outside of class hours:

• Scales, weigh boats and vortexes
• 500 sterile capped tubes with 25 mL LB – need about 10/student
• pipetman and 100 µL tips – need about 10 tips per student
• BZB1011 for use as positive control – stored in refrigerator
• 500 MacConkey plates – need about 10 per student
• 500 sterile swabs – need about 10 per student