Drugs

Drugs kdorfman Mon, 01/16/2012 - 15:07

Needed for

March 13 & 15: mussel lab 1

March 27 & 29: mussel lab 2

April 3 & 5: mussel lab 3

Acetyl Choline (Fisher AC159170050 5 gr, $13.92)

Serotonin (Fisher A550328M050 50 mg, $34.32)

Acetylcholine

Acetylcholine kdorfman Thu, 02/23/2012 - 15:57

Fisher AC159170050 5 gr, $13.92

MW = 181.7

Working concentration = 10^-4M = 0.01817 g/L


10 mL 100X stock

0.01817 g acetylcholine

10 mL ringers or seawater


12 mL 10X stock for students to make dilutions

1.2 mL 100X stock

Ringers or sea water to 12 mL

Aliquot 2 mL into 5 tubes


15 mL 1X working concentration

0.15 mL 100X stock

Ringers or sea water to 15 mL

aliquot 1 mL into 14 tubes

Serotonin

Serotonin kdorfman Thu, 02/23/2012 - 15:52

(Fisher A550328M050 50 mg, $34.32)

MW = 212.68

soluble up to 100 mg/mL in water

Working Concentration = 10^-5M

X M mM ┬ÁM mg/mL
1X 10^-5 0.01 10 0.0021268
10X 10^-4 0.1 100 0.021268
100X 10^-3 1 1000 0.21268
1000X 10^-2 10 2.1268

1.4 mL 1000X stock

=10 mM

3 mg (use the mg scale in 266A)

1.41 mL 1X ringers or sea water


12 mL 10X solution for students to make dilutions

0.12 mL 1000X stock

11.88 mL 1X Ringers or sea water

aliquot 2 mL into 5 tubes


15 mL 1X working concentration for student use

0.015 mL 1000x stock

15 mL Ringers or sea water

aliquot 1 mL into 14 tubes