On soil
On soil kdorfman Mon, 01/16/2012 - 15:43Arabidopsis planting guide - Plants on soil
Routine weekly planting to produce plant material for observation or DNA extraction
- Pots
- 6 pots in a big flat with no holes
- fill with clump-free potting soil, leaving some space at the rim
- Treat with Gnatrol for fungus gnats
- saturate with hot water the night before Teddi comes
- Arrange for Teddi to do Gnatrol treatment
- OR Heat in oven
- pre-heat oven to 225F (=~107C)
- Put soil-filled pots in aluminum lasagne pan
- Cover with foil
- insert thermometer in one pot near center
- Heat to 180F (=~82C)
- leave in oven for 30 min.
- leave covered until ready to plant
- Seeds for DNA
- Start a set 4 weeks before needed, another set 3 weeks before
- Columbia wild type (col-0)
- Scatter seeds on surface of soil (remoisten before if it is dry)
- Put on plastic cover
- Put in refrigerator, keep dark (this synchronizes germination)
- Transfer to growth chamber on the third day
- If no room in fridge, put seeds in microfuge tube, add water, refrigerate in dark 2 days, then plant
- Seeds for demo or experimental plants
- Put seeds in microfuge tube, water, refrigerate, keep dark
- 3rd day: pour into small beaker, add more water
- Deposit one seed to each corner of the pot, plus one in the middle with a transfer pipet.
- Transfer to growth chamber
- Growth Chamber
- 22C, 16H light, schedule continuous program
- Water bottom flat ~3x per week
- Remove cover after plants are well established.