Enzymes & kits
Enzymes & kits kdorfman Wed, 01/18/2012 - 23:28- Takara Ex-Taq
giant economy size: RR001B 4x 250U $590 at Clontech
$843 at Fisher
Fisher equivalent has this buffer: Tris-HCl 100mM, pH 9, KCl 500 mM, MgCl2 15 mM. Takara buffer is 20 mM MgCl2
try this? cheap Taq at http://www.bulldog-bio.com/bioreadyrtaq.html
Invitrogen 18080044 superscript III 200U/µL 10KU @ $259
Invitrogen 10777019 RNAse out 5KU @ $143
Invitrogen RNAse A Check supply.
Qiagen 79254 DNAse
includes RNase-free water and RDD for diluting the DNAseQiagin RNEasy 74904
- if needed RW1 1053394
- if needed RLT 79216
Quantifast 204054 400 rxns for q pcr
Zymo Clean & Concentrator kit
Oligo-dT Fisher FERSO131 Oligo dT 100 µM 60 µL @ ~$50. Dilute to 50µM!! (for emergencies: 50 µM oligo(dT) Fisher stockroom: C1101 0.5µg/µL x 40µL = 20µg)
2015 (S) Restriction list
2015 (S) Restriction list kdorfman Fri, 02/06/2015 - 16:03Enzyme | Cut Smart | NEB 1 | NEB 2 | NEB 3 | NEB 4 |
---|---|---|---|---|---|
Aat2 | 100 | 10 | 50 | 50 | |
Acc651 | 25 | 10 | 75 | 100 | |
Acl1 | 100 | 10 | 10 | 10 | |
AflII | 100 | 50 | 100 | 10 | |
Age1[^1] | 75 | 100 | 75 | 25 | |
AlwN1 | 100 | 10 | 100 | 50 | |
Apal1 | 100 | 100 | 100 | 10 | |
Asc1 | 100 | 10 | 10 | 10 | |
Ava1 | 100 | 10 | 100 | 25 | |
Ava2 | 100 | 50 | 75 | 10 | |
BamH1-HF | 100 | 100 | 50 | 10 | |
BciVI | 100 | 100 | 25 | 10 | |
Bgl2 | 10 | 10 | 10 | 100 | |
BmgB1 | 10 | 10 | 10 | 100 | |
BsaI | 100 | 75 | 75 | 100 | |
BspD1 | 100 | 25 | 75 | 50 | |
BspE1 | 10 | 10 | 10 | 100 | |
BsrB1 | 100 | 50 | 100 | 100 | |
BsrG1 | 25 | 25 | 100 | 100 | |
Cla1 | 100 | 10 | 50 | 50 | |
Dra1 | 100 | 75 | 75 | 50 | |
Eag1 | 10 | 10 | 25 | 100 | |
Ear1 | 100 | 50 | 10 | 10 | |
EcoR1 | 50 | 25 | 100 | 50 | |
EcoRV-HF | 100 | 25 | 100 | 100 | |
HaeIII | 100 | 50 | 100 | 25 | |
Hha1 | 100 | 25 | 100 | 100 | |
Hind3 | 50 | 25 | 100 | 50 | |
Hind3 | 50 | 25 | 100 | 50 | |
HinP1I | 100 | 100 | 100 | 100 | |
Hpa1 | 100 | 10 | 75 | 25 | |
Hpa2 | 100 | 100 | 50 | 10 | |
Kas1 | 100 | 50 | 100 | 50 | |
Mbo1 | 100 | 75 | 100 | 100 | |
Mlu1 | 25 | 10 | 50 | 100 | |
Msp1 | 100 | 75 | 100 | 50 | |
Nae1 | 100 | 25 | 25 | 10 | |
Nco1 | 100 | 100 | 100 | 100 | |
Nde1 | 100 | 75 | 100 | 100 | |
Nhe1-HF | 100 | 100 | 25 | 10 | |
Pst1 | 50 | 75 | 75 | 100 | |
Pvu1 | 10 | 10 | 25 | 100 | |
Pvu2 | 100 | 50 | 100 | 100 | |
Rsa1 | 100 | 25 | 50 | 10 | |
Sac1 | 100 | 100 | 50 | 10 | |
Sac2 | 100 | 10 | 100 | 10 | |
Sal1 | 10 | 10 | 10 | 100 | |
Sau3A1 | 100 | 100 | 50 | 10 | |
Sca1 | NR | NR | NR | 100 | |
SexA1 | 100 | 100 | 75 | 50 | |
Spe1 | 100 | 75 | 100 | 25 | |
Sph1 | 100 | 100 | 100 | 50 | |
SphI | |||||
SSpI | |||||
Sty1 | 10 | 10 | 25 | 100 | |
Sty1-HF | 100 | 25 | 100 | 25 | |
Xba1 | 100 | 10 | 100 | 75 | |
Xcm1 | 100 | 10 | 100 | 25 | |
Xho1 | 100 | 75 | 100 | 100 | |
Xma1 | 100 | 25 | 50 | 10 |
[^1] gone as of 9/16
2017S Restriction list
2017S Restriction list kdorfman Mon, 02/13/2017 - 17:11Enzyme | Cut Smart | NEB 1 | NEB 2 | NEB 3 | NEB 4 |
---|---|---|---|---|---|
Aat2 | 100 | 10 | 50 | 50 | |
Acc651 | 25 | 10 | 75 | 100 | |
Aci1 | 100 | 10 | 25 | 100 | |
Acl1 | 100 | 10 | 10 | 10 | |
AflII | 100 | 50 | 100 | 10 | |
Age1 | 75 | 100 | 75 | 25 | |
AlwN1 | 100 | 10 | 100 | 50 | |
Apal1 | 100 | 100 | 100 | 10 | |
Asc1 | 100 | 10 | 10 | 10 | |
Ava1 | 100 | 10 | 100 | 25 | |
Ava2 | 100 | 50 | 75 | 10 | |
BamHI-HF | 100 | 100 | 50 | 10 | |
BciVI | 100 | 100 | 25 | 10 | |
Bgl2 | 10 | 10 | 10 | 100 | |
BmgB1 | 10 | 10 | 10 | 100 | |
BmsAI | 100 | 50 | 100 | 100 | |
BsaI | 100 | 75 | 75 | 100 | |
BspD1 | 100 | 25 | 75 | 50 | |
BspE1 | 10 | 10 | 10 | 100 | |
BsrB1 | 100 | 50 | 100 | 100 | |
BsrG1 | 25 | 25 | 100 | 100 | |
Cla1 | 100 | 10 | 50 | 50 | |
Dra1 | 100 | 75 | 75 | 50 | |
Eag1 | 10 | 10 | 25 | 100 | |
Ear1 | 100 | 50 | 10 | 10 | |
EcoR1 | 50 | 25 | 100 | 50 | |
EcoRV-HF | 100 | 25 | 100 | 100 | |
HaeIII | 100 | 50 | 100 | 25 | |
Hha1 | 100 | 25 | 100 | 100 | |
Hind3 | 50 | 25 | 100 | 50 | |
Hind3 HF | 100 | 10 | 100 | 10 | |
HinP1I | 100 | 100 | 100 | 100 | |
Hpa1 | 100 | 10 | 75 | 25 | |
Hpa2 | 100 | 100 | 50 | 10 | |
Kas1 | 100 | 50 | 100 | 50 | |
KpnI-HF | 100 | 100 | 25 | 10 | |
Mbo1 | 100 | 75 | 100 | 100 | |
Mlu1 | 25 | 10 | 50 | 100 | |
Msp1 | 100 | 75 | 100 | 50 | |
Nae1 | 100 | 25 | 25 | 10 | |
Nco1 | 100 | 100 | 100 | 100 | |
Nde1 | 100 | 75 | 100 | 100 | |
Nhe1-HF | 100 | 100 | 25 | 10 | |
Pst1 | 50 | 75 | 75 | 100 | |
Pvu1 | 10 | 10 | 25 | 100 | |
Pvu2 | 100 | 50 | 100 | 100 | |
Rsa1 | 100 | 25 | 50 | 10 | |
Sac1 | 100 | 100 | 50 | 10 | |
Sal1 | 10 | 10 | 10 | 100 | |
Sau3A1 | 100 | 100 | 50 | 10 | |
Sca1 | NR | NR | NR | 100 | |
SexA1 | 100 | 100 | 75 | 50 | |
Sma1 | 100 | 10 | 10 | 10 | |
Spe1 | 100 | 75 | 100 | 25 | |
Sph1 | 100 | 100 | 100 | 50 | |
SSpI | 50 | 50 | 100 | 50 | |
Sty1 | 10 | 10 | 25 | 100 | |
Sty1-HF | 100 | 25 | 100 | 25 | |
Xba1 | 100 | 10 | 100 | 75 | |
Xcm1 | 100 | 10 | 100 | 25 | |
Xho1 | 100 | 75 | 100 | 100 |