P. m. medium
P. m. medium kdorfman Sat, 02/04/2012 - 17:40Growth medium of axenic P. multimicromucleatum culture
from http://protistology.ifmo.ru/num4_2/tan.pdf
Ingredient | conc | MW | supplier | |
---|---|---|---|---|
L-a-Phosphatidylethanolamine (=cephalin) | 0.2 | g/L | Sigma P7943-100MG $352, Fisher 50-750-5972 50g $125 | |
Proteose peptone | 10 | g/L | ||
Tryptone | 0.5 | g/L | ||
Ribonucleic acid | 1.0 | g/L | Sigma R6625-25G $48.90 | |
Magnesium sulfate heptahydrate | 4.2 | mM | ||
Stigmasterol | 0.006 | mM | 412 | =0.00247 g/L Fisher 50-014-46501 1 g $39.30 |
D_Pantothenic acid hemicalcium salt | 0.02 | mM | 238 | =4.76 mg/L 50mg/mL H2O SigmaP2250-5G 24.50 |
Nicotinamide | 0.04 | mM | 122 | Fisher AC12827-1000 100g $14.63 |
Pyridoxal hydrochloride | 0.25 | mM | 203.63 | Fisher AC35271-0010 1g $34.69 need 51 mg/L |
Pyridoxamine dihydrochloride | 0.01 | mM | 241.12 | Fisher AC43625-0010 1g $80.94 need 2.41 mg/L |
Riboflavin | 0.01 | mM | 376.37 | Fisher AC13235-0250 25 g $24.18 need 3.7 mg/L |
Folic acid | 0.06 | mM | 441 | =0.026g/L Fisher 10g AC21663-0100 $13.54 soluble 50mg/mL in 1M NaOH |
Thiamine hydrochloride | 0.04 | mM | 337.27 | Fisher AC14899-0100 10 g $29.79 need 13.5 mg/L |
Biotin | 0.01 | μM | 244.31 | =0.0000024g/L; 100mg/0.41L = 1mM. soluble 50 mg/mL in 2M NH4OH Fisher 50-014-20193 100 mg $30.12 |
Lipoic acid | 0.24 | μM | 206.33 | Fisher 1g BP2682-1 $45.92 50mg/mL EtOH |
NH4OH 500mL Fisher ICN19385490 MP Biomedicals No.:0219385490 $34.49 ~28% MW = 35 so solution is ~1M
In other studies that require stringent, defined conditions, stigmasterol and either oleate, monolein, or synthetic dioleoyl PsE are the only lipids included in the chemically defined medium
The Kit includes
- 250 mL of the fresh Paramecium Culture at ~3,000 cells/ml in a diluted Tübingen E3 medium for Zebrafish embryos
- 250 mL of Paramecium Culture Medium concentrate for preparation of 20.0 L of growth medium
- 50 mL of Paramecium Food for high density cultures, sufficient for preparation of 5.0 L
Suggested schedule of feeding Zebrafish larvae with paramecium
1 kit $75 (shipping and handling included)