LB amp

LB amp kdorfman Wed, 02/08/2012 - 15:40

LB ampicilin plates for transformation

48 plates x 25 mL/plate = 1200 mL

Make 1500 mL:

25 g LB + 15 g agar /liter and 50 µg/mL ampicillin

http://wahoo.nsm.umass.edu/content/ampicillin

37.5 g LB in 1500 mL water in a 2L flask

stir to dissolve, leave stir bar in.

add 22.5 g agar

Autoclave 50 min. Include PourBoy tubing if necessary, and 2 flasks of water.

Cool till handle-able

Add 1500 µL 50 mg/mL amp

Stir

Dispense 25 mL/plate