Sequencing

Sequencing kdorfman Mon, 04/02/2012 - 17:50

Primers for the 5'

  • Splac2 25mer GAATTCACTGGCCGTCGTTTTACAA

  • Sp1 22mer ACACAACCTTTCCTCTCAACAA

Primers for the 3'

  • Spep1 19mer GACACTCAGAATACTATTC

  • Sp6 23mer TGACCACATCCAAACATCCTCTT

Melting Temps for sequencing primers

  • Splac2 60.1C

  • Sp1 50.6C

  • Sp6 54.9C

  • Spep1 44.8C

sequencing results

sequencing results kdorfman Tue, 04/10/2012 - 18:21

Get Ape (A Plasmid Editor) for Mac to read the sequences (if it isn't installed already)

http://biologylabs.utah.edu/jorgensen/wayned/ape/

57d-3klcae-sp6_c05.seq
57d-3ckaoc-sp6_g06.seq
57d-3wahai-sp6_e04.seq
57d-3wahai-spep1_d04.seq
57d-3klcam-sp6_g05.seq
57d-3klcam-spep1_f05.seq
57d-5klcam-sp1_d05.seq
57d-5klcam-splac2_e05.seq
57d-5gemed-sp1_b06.seq
57d-5gemed-splac2_c06.seq
57d-3lacedd-spep1_f04.seq
57d-5lacedd-sp1_h04.seq
57d-5ckaoc-splac2_a07.seq
57d-5ckaoc-sp1_h06.seq
57d-3ckaoc-spep1_f06.seq
57d-3lacedd-sp6_g04.seq
57d-3klcas-sp6_a06.seq
57d-3klcas-spep1_h05.seq
57d-3ckaoc-sp6_g06.seq
57d-3ckaoc-spep1_f06.seq
57d-3ckaza-sp6_e07.seq
57d-3ckaza-spep1_d07.seq
57d-3gemjq-sp6_e06.seq
57d-3gemjq-spep1_d06.seq
57d-3klcae-spep1_b05.seq
57d-5ckaac-sp1_b07.seq
57d-5lacedd-splac2_a05.seq