Formaldehyde fix

Formaldehyde fix kdorfman Thu, 07/05/2012 - 20:55

Make in fume hood 3.7% formaldehyde
0.5% Triton X
in PBS

Formaldehyde stock: 37%
Triton X stock: 10%

To make mL fixative
add: mL 37% formaldehyde
add: mL 10X PBS
and: mL 10% Triton-X

To fix the adherent cells (still in hood):