2012
2012 kdorfman Mon, 09/17/2012 - 17:57First thing, the morning before:
- Pick a colony
- Grow in 3 mL LB-Kan
Last thing, the day before: 100 µL culture into 40 mL (each w/ Kan)
- LB
- Lac 0.25 M
- 20 mL LB
- 20 mL Lac 0.5 M
- IPTG 0.1mM
- 20 mL LB
- 20 mL 0.2 mM IPTG
- Glu 0.25 M
- 20 mL LB
- 20 mL 0.5 M glu
- Gal 0.25 M
- 20 mL LB
- 20 mL 0.5 mL gal
- Mal 0.25 M
- 20 mL LB
- 20 mL 0.5 M mal
- IPTG 0.1 mM + glu 0.25 M
- 20 mL 0.2 mM IPTG
- 20 mL 0.5 M glu
- IPTG 0.1 mM + mal 0.125 M
- 20 mL 0.2 mM IPTG
- 10 mL LB
- 10 mL 0.5 M mal
- Lac 0.25 M + glu 0.25 M
- 20 mL 0.5M lac
- 20 mL 0.5 M glu
- Lac 0.25 M + mal 0.125 M
- 20 mL 0.5M lac
- 10 mL LB
- 10 mL 0.5 M mal
- Gal 0.25 M + glu 0.25 M
- 20 mL 0.5 M gal
- 20 mL 0.5 M glu
- Gal 0.25 M + mal 0.125 M
- 20 mL 0.5 M gal
- 10 mL LB
- 10 mL 0.5 M mal
Morning of:
- Aliquot ~4 mL each culture into plastic tubes.
- One set per 4 tables.
- ? save for next lab in fridge?
Media for Lac I
Media for Lac I kdorfman Mon, 09/17/2012 - 18:10LB (150 mL)
LB +
0.1mM IPTG (60 mL)
0.5 M lac (60 mL)
0.5 M glu (80 mL)
0.5 M gal (60 mL)
0.5 M mal (40 mL)
Make enough for Lac operon I and II:
150 mL each + 50µg/mL Kan
lac = 27 g
gal = 13.5 g
mal = 27 g
IPTG = 0.3 mL 100mM stock
200 mL glu = 18 g
Kan calculation here: