sterilize seeds
sterilize seeds kdorfman Fri, 05/24/2013 - 18:33sterilize seeds in 0.5 mL 70% EtOH, 0.05% TritonX-100, ~3 min, mild agitation.
Let seeds settle, remove liquid
Replace with sterile water, 3X
Replace water with sterile 0.1% agar, 3X
plate heavily in a line on flattened cellophane (wet it if necessary)
stack plates flat
cover in foil, refrigerate, 48 hours