ATP

10 µM (=5.731 mg/L) ATP in HBS

Use ATP aliquots from Lab 6.1

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10 µM ATP, Calcium-free

Lab 6.2: 0.75 mL per coverslip x 2 coverslips x 8 groups = 12 mL

Make 15 mL

• 15 µL 10 mM ATP stock
• in 15 mL Ca- HBS (1 µL 10 mM ATP stock per 1 mL HBS.)

8 1.6 mL aliquots for Lab 6.2