09/03/14

09/03/14 kdorfman Fri, 09/12/2014 - 14:53

Lab 1: Bioinformatics W 9/3/14

Update computers if necessary:

username: radmind

password: [none - hit enter twice]

09/05/14

09/05/14 kdorfman Fri, 09/12/2014 - 14:55

Lab 2: Identify targeting sequence and construct design

F 9/5/14

Update computers if necessary.

Order primers on Kate's procard.

09/10/14

09/10/14 kdorfman Fri, 09/12/2014 - 14:59

Lab 3.1

Micropipetting (exercise from Gene & Genome Analysis)

Isolating genomic DNA

Equipment

small centrifuges
vortex
waste buckets
tips
microfuge tubes

Solutions

phusion kit
.
.

09/12/14

09/12/14 kdorfman Fri, 09/12/2014 - 15:03

F 9/12/14

Lab 3.2: PCR

Phusion kit
aliquoted buffer

09/17/14

09/17/14 kdorfman Fri, 09/12/2014 - 15:05

W

Lab 3.3: Analyze PCR with agarose gel, purify DNA fragment, Sent DNA for sequencing

Equipment * . * .

Solutions * . * .

10/1/14

10/1/14 kdorfman Fri, 09/12/2014 - 15:11

Lab 3.7

Lab 3.7 kdorfman Fri, 09/12/2014 - 15:11

W 10/1/14

(4.1 same day)

Analyze sequencing data

Lab 4.1

Lab 4.1 kdorfman Fri, 09/12/2014 - 15:49

(Lab 3.7 same day)

Set up LR cloning reaction

Lab 3.4

Lab 3.4 kdorfman Fri, 09/12/2014 - 15:06

F 9/19/14

Set up and transform BP cloning reaction

Lab 3.5

Lab 3.5 kdorfman Fri, 09/12/2014 - 15:09

W 9/24/14

Mini-prep DNA, restriction digest, gel

Lab 3.6

Lab 3.6 kdorfman Fri, 09/12/2014 - 15:10

F 9/26/14

Analyze restriction pattern, measure DNA concentration, send DNA for sequencing

Lab 4.2

Lab 4.2 kdorfman Fri, 09/12/2014 - 15:13

F 10/3/14

Transform LR recombination reaction into E. coli

Lab 4.3

Lab 4.3 kdorfman Fri, 09/12/2014 - 15:14

W 10/8/14

Mini-preps, restriction digest, gel

Lab 4.4

Lab 4.4 kdorfman Fri, 09/12/2014 - 15:16

F 10/10/14

Analyze restriction pattern, transform positive clone into E. coli

Lab 5.1

Lab 5.1 kdorfman Fri, 09/12/2014 - 15:17

W 10/15/14

Amplify DNA for plant transformation

Lab 5.2

Lab 5.2 kdorfman Fri, 09/12/2014 - 15:18

F 10/17/14

(Lab 6.1 same day)

Measure DNA concentration, send for sequencing

Lab 5.3

Lab 5.3 kdorfman Fri, 09/12/2014 - 15:22

W 10/22/14

(Lab 6.2 same day)

Analyze sequencing data

Lab 6.1

Lab 6.1 kdorfman Fri, 09/12/2014 - 15:20

F 10/17/14

(Lab 5.2 same day)

Propagating Moss tissue

Lab 6.2

Lab 6.2 kdorfman Fri, 09/12/2014 - 15:23

W 10/22/14

(same day as Lab 5.3)

Protoplasting moss tissue

Lab 6.3

Lab 6.3 kdorfman Fri, 09/12/2014 - 15:24

F 10/24/14

Acquire imaging of protoplasts

Lab 6.4

Lab 6.4 kdorfman Fri, 09/12/2014 - 15:25

W 10/29/14

(see also Lab 7.1)

Analyze organelle movement

Lab 6.5

Lab 6.5 kdorfman Fri, 09/12/2014 - 15:27

F 11/31/14

Analyze additional organelle motility

Lab 7.1 (first)

Lab 7.1 (first) kdorfman Fri, 09/12/2014 - 15:26

W 10/29/14

(Lab 6.4 same day)

Propagate moss as in Lab 6.1

Lab 7.1 (repeat)

Lab 7.1 (repeat) kdorfman Fri, 09/12/2014 - 15:31

W 11/19/14

Propagate moss as in Lab 6.1 (2nd time)

Lab 7.2 (first)

Lab 7.2 (first) kdorfman Fri, 09/12/2014 - 15:27

W 11/5/14

Moss transformation

Lab 7.2 (repeat)

Lab 7.2 (repeat) kdorfman Fri, 09/12/2014 - 15:32

W 11/26/14

Moss transformation (2nd time)

Lab 7.3 (first)

Lab 7.3 (first) kdorfman Fri, 09/12/2014 - 15:29

F 11/7/14

Acquire imaging of 2-day old transformed protoplasts

Lab 7.4

Lab 7.4 kdorfman Fri, 09/12/2014 - 15:30

F 11/14/14

Acquire images of 9-day old transformed protoplasts