2014

Mixtures needed to grow 50 mL cultures for qualitative experiment

Need an additional ~120 mL of each sugar for quantitative student experiments the next week. (24 5-mL aliquots each)

LB-sugar recipes

medium	LB	lac	IPTG	glu	gal	mal
a	50
b	25	25
c	25		25
d	25			25
e	25				25
f	25					25
g			25	25
h	12.5		25			12.5
i		25		25
j	12.5	25				12.5
k				25	25
l	12.5				25	12.5
Totals	212.5	75	75	100	75	62.5

Plus at least 100 mL for student experiments

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To make 25 mL for each culture (6 sets of 12 4-mL cultures):

medium	LB	lac	IPTG	glu	gal	mal
a	25
b	12.5	12.5
c	12.5		12.5
d	12.5			12.5
e	12.5				12.5
f	12.5					12.5
g			12.5	12.5
h	12.5		12.5			12.5
i		12.5		12.5
j	6.25	12.5				6.25
k				12.5	12.5
l	6.25				12.5	6.25
Totals	106,25	37.5	37.5	50	37.5	31.25