2016 schedule
2016 schedule kdorfman Wed, 09/21/2016 - 20:06Lab 1 1 DAPI slide per pair
Lab 2
same DAPI slide as Lab 1
1 H&E slide from histology
Lab 3 Numerical aperture . same DAPI slide
Lab 4 Resolution.
1 slide newly stained phalloidin actin. Must be bright
1 slide fluorescent beads(PS-Speck Microscope Point Source Kit P7220 from Molecular Probes)
Lab 5 Fluorescence. 1 new, bright 3-color stained slide (DAPI, Phalloidin-actin, FITC microtubules)
Lab 6 Dry lab
Lab 7 Labeling Cells 9/28/16
3 fixed LL coverslips
1 3t3 fixed coverslip
1 live LL coverslip for students to fix
paraglut
forceps, mounting medium, pasteur pipettes
Lab 8 - Organelles 10/3/16
Golgi - anti-58K, mouse, formaldehyde fixation
Kinetochore - anti Hec1 - MeOH fixation 1:200 mouse
ER anti-calnexin, paraformaldehyde fixation 1:200
centrosome - anti gamma-tubulin, paraglut, mouse 1:100
nuclear envelope anti LAP2 - MeOH fixation 1:100 mouse
lysosomes
alpha actinin - mouse 1:200 formaldehyde fixation
Lab 8 Practical (and pipet practice)- 10/5
Lab 9 - Imaging Live Cells 10/11 TUESDAY Live cells on mattek
unknown GFP tagged proteins
Thaw GFP lines Wednesday
Split Thursday (~1/4)
Plate Sunday on Mateks for Tuesday
- Thaw
- GFP tubulin
- actin thaw
- EB1 - thaw
- Nucleofect: EGFP-H2B
- split LL parentals on Sunday, HEAVY
- nucleofect & plate Monday by noon
Lab 10 10/12
Single cells to whole organisms
order protists
induce GFP E. coli
CRISPR: Transform guide RNA plasmid into bacteria
Lab 11.1 Motility I 10/17
- thaw B16, 3t3 Thursday 10/13/16 (late in the day)
- plate 9 MatTeks each Fri 10/14/16:
- 3t3
- actin GFP LL
- B16
Lab 11.2 Motility II 10/19
Lab 11.3 Motility III 10/24
Motility presentations 10/26
CRISPR: prepare Cas9/guide RNA plasmid DNA 10/31
CRISPR: prepare repair DNA cassette (PCR, clean up) 11/2
CRISPR: Add Cas9 plasmid + repair DNA to cells 11/7
Lab 13.1 - Cell Signaling 11/9
Lab 13.2 - Cell Signaling 2 11/14
11/16 Wed = Friday schedule
Discussion of independent projects 11/28
Projects 11/30 - 12/14
Final exam period: Final project presentation